Upon screen execution and completion, the following data will be shared: We then undergo a standard data processing pipeline with extensive QC (~4 weeks) We use two cell sets (PR500, PR300+) which each undergo a 5-day treatment (~2 weeks)Īfter cell treatment, we lyse the cells, amplify the barcodes, and detect them using a bead-based Luminex system (~4 weeks) Next, we add the cells directly on top of the compound in 384-well plates. Once all compounds have been received, we make them into Assay Ready Plates using the Broad Institute’s Compound Management group’s automated liquid handling capabilities (~2 weeks) If you don’t hear from us while the assay is running that means everything is proceeding on-time! Our typical assay workflow is as follows: If for any reason we are unable to meet that deadline, we will let you know as soon as possible. Users may submit a small number of compounds (1000 compounds) against our standard PR500 cell linesĬollaborators may select timepoint (up to 5 days), top dose and number of doses, biological replicates.ĭata release occurs approximately 3 months after the compound submission deadline. PRISM projects typically fall into one of three categories which offer different degrees of customization: Intellectual property will be jointly filed on jointly discovered inventions Key points of collaboration include:Ĭompound structures do not need to be sharedĭata may be published only when the collaborator agrees We work with collaborators on scientifically engaging projects as part of a collaboration. We do not offer a Fee-for-Service model for PRISM. Please review the details of our process below and send us a message if you’re interested in collaborating with us: Over 29,000 compounds have been screened in 500+ PRISM cell lines since 2015. PRISM has worked with 200+ collaborators from academic institutions, pharmaceutical companies, and biotechnology startups.
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